Multi-cell biochemical assays and single cell fluorescence measurements revealed that the elongation rate of Polymerase II (PolII) in eukaryotes varies largely across different cell types and genes.

Being able to see precisely when various genes are turned on and off, as well as spatially visualizing the whole transcriptome at once, has been challenging for single-cell analysis. However, now, ...

The fundamental process of gene transcription can be reconstituted in vitro, but an understanding of how it operates and how it is regulated in vivo is lacking. One hypothesis suggests that, rather ...

A newly developed mouse model enables real-time visualization of RNA Polymerase II (RNAP2) during DNA transcription, as reported by researchers from Science Tokyo. The team engineered mice to produce ...