Production of endonucleolytic double-strand DNA breaks requires separate strand cleavage events. Although catalytic mechanisms for simple, dimeric endonucleases are known, there are many complex ...

Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...

For the first time scientists have been able to film, in real time, the nanoscale interaction of an enzyme and a DNA strand from an attacking virus. Researchers from the University of Cambridge have ...

For the first time scientists have been able to film, in real time, the nanoscale interaction of an enzyme and a DNA strand from an attacking virus. Researchers have used a revolutionary Scanning ...

Homologous recombination is an ancient process that functions to conserve genetic integrity and create genetic diversity. It relies on the formation of Holliday junctions — four-stranded DNA ...