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45:58
YouTube
the bumbling biochemist
Protein chromatography - types, tips, & example workflow for protein purification
The basic idea with this is that you can flow a solution containing the protein through columns filled with little beads (resin) and the proteins will get separated based on how they interact with the resin on their journey. Different proteins have different properties, so they will travel faster or slower (in SEC) or “stick” or “not ...
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