RT-qPCR (Reverse Transcription/Real Time - quantitative Polymerase Chain Reaction) theory & practice
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YouTubethe bumbling biochemist
RT-qPCR (Reverse Transcription/Real Time - quantitative Polymerase Chain Reaction) theory & practice
Quantitative PCR (qPCR) is a way to “count copies” of specific pieces of DNA - and when those specific pieces are DNA copies of RNA (like the RNA copies of protein recipes) we call it Reverse Transcription (RT) qPCR - RT can also stand for Real Time, which refers to the measuring - the products are detected as they’re made because of the ...
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