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A molecule of double-stranded DNA was cleaved with restriction nucleases, and the resulting products were separated by gel electrophoresis (Figure Q 10-11 ). DNA fragments of known sizes were electrophoresed on the same gel for use as size markers (left lane). The size of the DNA markers is given in kilobase pairs (kb), where 1 kb= 1000 nucleotide pairs. Using the size markers as a guide, estimate the length of each restriction fragment obtained. From this information, construct a map of the ori
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A preparation of circular plasmid DNA was digested with three different combinations of restriction enzymes. The patterns of the fragments following electrophoresis are shown below. Construct a restriction map of this plasmid DNA.1. EcoRI 9.0kb2. BamHI 6.7kb3. EcoRI BamHI 1.3kb, 2.0kb, 4.0kb
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BamH1 is a restriction enzyme which recognizes the sequence- 5'-GGATCC-3'. The restriction activity of this enzyme is between G and G. A. Construct the palindrome for the above sequence. - Biology | Shaalaa.com
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